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goat anti pcsk9  (R&D Systems)


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    R&D Systems goat anti pcsk9
    Goat Anti Pcsk9, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+pcsk9/Mouse%2FRat+Proprotein+Convertase+9%2FPCSK9+Antibody/us12577546-828-12-14
    Average 93 stars, based on 25 article reviews
    goat anti pcsk9 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Membrane:

    Article Title: A compact, high-accuracy Cas9 with a dinucleotide PAM for in vivo genome editing
    Article Snippet: The separated bands were transferred onto a PVDF membrane and blocked with 5% Blocking-Grade Blocker solution (Bio-Rad) for 2 hours at room temperature. .. Next, the membrane was incubated with rabbit anti-GAPDH (Abcam ab9485, 1:2,000) or goat anti-PCSK9 (R&D Systems AF3985, 1:400) antibodies overnight. .. Membranes were washed in TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Bio-Rad 1706515, 1:4,000), and donkey anti-goat (R&D Systems HAF109, 1:2,000) secondary antibodies for 2 hours at room temperature.

    Article Title: Programmable DNA base editing by Nme2Cas9-deaminase fusion proteins
    Article Snippet: The separated bands were transferred onto a PVDF membrane and blocked with 5% Blocking-Grade Blocker solution (Bio-Rad) for 2 hours at room temperature. .. Next, the membrane was incubated with rabbit anti-GAPDH (Abcam ab9485, 1:2,000) or goat anti-PCSK9 (R&D Systems AF3985, 1:400) antibodies overnight. .. Membranes were washed in TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Bio-Rad 1706515, 1:4,000), and donkey anti-goat (R&D Systems HAF109, 1:2,000) secondary antibodies for 2 hours at room temperature.

    Incubation:

    Article Title: A compact, high-accuracy Cas9 with a dinucleotide PAM for in vivo genome editing
    Article Snippet: The separated bands were transferred onto a PVDF membrane and blocked with 5% Blocking-Grade Blocker solution (Bio-Rad) for 2 hours at room temperature. .. Next, the membrane was incubated with rabbit anti-GAPDH (Abcam ab9485, 1:2,000) or goat anti-PCSK9 (R&D Systems AF3985, 1:400) antibodies overnight. .. Membranes were washed in TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Bio-Rad 1706515, 1:4,000), and donkey anti-goat (R&D Systems HAF109, 1:2,000) secondary antibodies for 2 hours at room temperature.

    Article Title: All-in-One Adeno-associated Virus Delivery and Genome Editing by Neisseria meningitidis Cas9 in vivo
    Article Snippet: The separated bands were transferred onto PVDF membrane and blocked with 5% Blocking-Grade Blocker solution (Bio-Rad) for 2 hours at room temperature. .. Membranes were incubated with rabbit anti-GAPDH (Abcam ab9485, 1:2000) or goat anti-PCSK9 (R&D Systems AF3985, 1:400) antibodies overnight at 4 °C. .. Membranes were washed five times in TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Bio-Rad 1706515, 1:4000), and donkey anti-goat (R&D Systems HAF109, 1:2000) secondary antibodies for 2 hours at room temperature.

    Article Title: All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo
    Article Snippet: The separated bands were transferred onto PVDF membrane and blocked with 5% Blocking-Grade Blocker solution (Bio-Rad) for 2 h at room temperature. .. Membranes were incubated with rabbit anti-GAPDH (Abcam ab9485, 1:2000) or goat anti-PCSK9 (R&D Systems AF3985, 1:400) antibodies overnight at 4 °C. .. Membranes were washed five times in TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Bio-Rad 1,706,515, 1:4000) and donkey anti-goat (R&D Systems HAF109, 1:2000) secondary antibodies for 2 h at room temperature.

    Article Title: Programmable DNA base editing by Nme2Cas9-deaminase fusion proteins
    Article Snippet: The separated bands were transferred onto a PVDF membrane and blocked with 5% Blocking-Grade Blocker solution (Bio-Rad) for 2 hours at room temperature. .. Next, the membrane was incubated with rabbit anti-GAPDH (Abcam ab9485, 1:2,000) or goat anti-PCSK9 (R&D Systems AF3985, 1:400) antibodies overnight. .. Membranes were washed in TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Bio-Rad 1706515, 1:4,000), and donkey anti-goat (R&D Systems HAF109, 1:2,000) secondary antibodies for 2 hours at room temperature.



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    Image Search Results


    Hepatic mRNA and protein expression of Pcsk9 and Anxa2 genes after 20 weeks on HFD. The hepatic expression was assessed at mRNA and protein levels by qPCR/ELISA for PCSK9 ( A, B ) and qPCR/Western blot for ANXA2 ( C, D ). The mRNA level was calculated with 2 − ΔΔ Ct method. The relative protein level on the blots was determined by ImageJ, using actin as an internal control. Values are reported as mean ± SD; n = 5 in each group. The p value was determined by unpaired T-test. * p < 0.05, ** p < 0.01, *** p < 0.001

    Journal: Nutrition & Metabolism

    Article Title: High fat diet and PCSK9 knockout modulates lipid profile of the liver and changes the expression of lipid homeostasis related genes

    doi: 10.1186/s12986-023-00738-z

    Figure Lengend Snippet: Hepatic mRNA and protein expression of Pcsk9 and Anxa2 genes after 20 weeks on HFD. The hepatic expression was assessed at mRNA and protein levels by qPCR/ELISA for PCSK9 ( A, B ) and qPCR/Western blot for ANXA2 ( C, D ). The mRNA level was calculated with 2 − ΔΔ Ct method. The relative protein level on the blots was determined by ImageJ, using actin as an internal control. Values are reported as mean ± SD; n = 5 in each group. The p value was determined by unpaired T-test. * p < 0.05, ** p < 0.01, *** p < 0.001

    Article Snippet: Primary antibodies were incubated overnight at 4 °C in 1:100 dilution (monoclonal mouse anti-CD36, clone: FA6-152, Abcam, UK; monoclonal rabbit anti-LDLR, clone: SJ0197, ThermoFisher; monoclonal rabbit anti-Annexin A2, clone: JA42-30, ThermoFisher; polyclonal goat anti-PCSK9, Sigma; monoclonal mouse anti-PCSK9, clone: 2F1, ThermoFisher).

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

    Lipid accumulation in the livers of PCSK9 −/− mice on control diet. Lipid composition of the liver was examined by HPLC–MS/MS. The concentration of TG, DG and CER subtypes ( A, F, K ), their total amount ( B, G, L ), the proportion of saturated and monounsaturated TGs, DGs and CERs to total amount ( C, D, H, I, M, N ) and the ratio of monounsaturated and saturated lipids were determined ( E, J, O ). Values are reported as mean ± SD; n = 5 in each group. The p value was determined by multiple T-test ( A, F, K ) or unpaired T-test (all others). * p < 0.05

    Journal: Nutrition & Metabolism

    Article Title: High fat diet and PCSK9 knockout modulates lipid profile of the liver and changes the expression of lipid homeostasis related genes

    doi: 10.1186/s12986-023-00738-z

    Figure Lengend Snippet: Lipid accumulation in the livers of PCSK9 −/− mice on control diet. Lipid composition of the liver was examined by HPLC–MS/MS. The concentration of TG, DG and CER subtypes ( A, F, K ), their total amount ( B, G, L ), the proportion of saturated and monounsaturated TGs, DGs and CERs to total amount ( C, D, H, I, M, N ) and the ratio of monounsaturated and saturated lipids were determined ( E, J, O ). Values are reported as mean ± SD; n = 5 in each group. The p value was determined by multiple T-test ( A, F, K ) or unpaired T-test (all others). * p < 0.05

    Article Snippet: Primary antibodies were incubated overnight at 4 °C in 1:100 dilution (monoclonal mouse anti-CD36, clone: FA6-152, Abcam, UK; monoclonal rabbit anti-LDLR, clone: SJ0197, ThermoFisher; monoclonal rabbit anti-Annexin A2, clone: JA42-30, ThermoFisher; polyclonal goat anti-PCSK9, Sigma; monoclonal mouse anti-PCSK9, clone: 2F1, ThermoFisher).

    Techniques: Tandem Mass Spectroscopy, Concentration Assay

    Hepatic gene expression of Pcsk9 and Anxa2 genes in PCSK9 −/− mice on control diet. The hepatic expression was assessed at mRNA and protein levels by qPCR/ELISA for PCSK9 ( A, B ) and qPCR/Western blot for (ANXA2; C, D ). The mRNA level was calculated with 2 − ΔΔ Ct method. The relative protein level on the blots was determined by ImageJ, using actin as an internal control. Values are reported as mean ± SD; n = 5 in each group. The p value was determined by multiple T-test ( A and B ). * p < 0.05, ** p < 0.01, *** p < 0.001

    Journal: Nutrition & Metabolism

    Article Title: High fat diet and PCSK9 knockout modulates lipid profile of the liver and changes the expression of lipid homeostasis related genes

    doi: 10.1186/s12986-023-00738-z

    Figure Lengend Snippet: Hepatic gene expression of Pcsk9 and Anxa2 genes in PCSK9 −/− mice on control diet. The hepatic expression was assessed at mRNA and protein levels by qPCR/ELISA for PCSK9 ( A, B ) and qPCR/Western blot for (ANXA2; C, D ). The mRNA level was calculated with 2 − ΔΔ Ct method. The relative protein level on the blots was determined by ImageJ, using actin as an internal control. Values are reported as mean ± SD; n = 5 in each group. The p value was determined by multiple T-test ( A and B ). * p < 0.05, ** p < 0.01, *** p < 0.001

    Article Snippet: Primary antibodies were incubated overnight at 4 °C in 1:100 dilution (monoclonal mouse anti-CD36, clone: FA6-152, Abcam, UK; monoclonal rabbit anti-LDLR, clone: SJ0197, ThermoFisher; monoclonal rabbit anti-Annexin A2, clone: JA42-30, ThermoFisher; polyclonal goat anti-PCSK9, Sigma; monoclonal mouse anti-PCSK9, clone: 2F1, ThermoFisher).

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

    Hepatic gene expression of Cd36 and Ldlr genes in PCSK9 −/− mice. Hepatic expression at mRNA level was measured by qPCR and calculated with 2 − ΔΔ Ct method ( A, C ). The relative protein level on the blots was determined by ImageJ, using actin as an internal control ( B, D ). Values are reported as mean ± SD; n = 5 in each group. The p value was determined by multiple T-test ( A and B ). * p < 0.05, ** p < 0.01, *** p < 0.001

    Journal: Nutrition & Metabolism

    Article Title: High fat diet and PCSK9 knockout modulates lipid profile of the liver and changes the expression of lipid homeostasis related genes

    doi: 10.1186/s12986-023-00738-z

    Figure Lengend Snippet: Hepatic gene expression of Cd36 and Ldlr genes in PCSK9 −/− mice. Hepatic expression at mRNA level was measured by qPCR and calculated with 2 − ΔΔ Ct method ( A, C ). The relative protein level on the blots was determined by ImageJ, using actin as an internal control ( B, D ). Values are reported as mean ± SD; n = 5 in each group. The p value was determined by multiple T-test ( A and B ). * p < 0.05, ** p < 0.01, *** p < 0.001

    Article Snippet: Primary antibodies were incubated overnight at 4 °C in 1:100 dilution (monoclonal mouse anti-CD36, clone: FA6-152, Abcam, UK; monoclonal rabbit anti-LDLR, clone: SJ0197, ThermoFisher; monoclonal rabbit anti-Annexin A2, clone: JA42-30, ThermoFisher; polyclonal goat anti-PCSK9, Sigma; monoclonal mouse anti-PCSK9, clone: 2F1, ThermoFisher).

    Techniques: Expressing

    Co-localization of PCSK9 with LDLR, CD36 and ANXA2. HepG2 cells were examined for PCSK9 secretion 16 and 40 h after serum depletion ( A , n = 3). Cellular localization of PCSK9, LDLR, CD36 and ANXA2 proteins was examined by laser scanning confocal microscopy ( B ). Co-localization rate was used to quantify the degree of co-localization ( C , n = 5). The scale bar has a width of 5 µm. Values are reported as mean ± SD. The p value was determined by unpaired T-test ( A ) or one-way ANOVA combined with Tukey’s multiple comparisons test ( C ). * p < 0.05, ** p < 0.01, *** p < 0.001

    Journal: Nutrition & Metabolism

    Article Title: High fat diet and PCSK9 knockout modulates lipid profile of the liver and changes the expression of lipid homeostasis related genes

    doi: 10.1186/s12986-023-00738-z

    Figure Lengend Snippet: Co-localization of PCSK9 with LDLR, CD36 and ANXA2. HepG2 cells were examined for PCSK9 secretion 16 and 40 h after serum depletion ( A , n = 3). Cellular localization of PCSK9, LDLR, CD36 and ANXA2 proteins was examined by laser scanning confocal microscopy ( B ). Co-localization rate was used to quantify the degree of co-localization ( C , n = 5). The scale bar has a width of 5 µm. Values are reported as mean ± SD. The p value was determined by unpaired T-test ( A ) or one-way ANOVA combined with Tukey’s multiple comparisons test ( C ). * p < 0.05, ** p < 0.01, *** p < 0.001

    Article Snippet: Primary antibodies were incubated overnight at 4 °C in 1:100 dilution (monoclonal mouse anti-CD36, clone: FA6-152, Abcam, UK; monoclonal rabbit anti-LDLR, clone: SJ0197, ThermoFisher; monoclonal rabbit anti-Annexin A2, clone: JA42-30, ThermoFisher; polyclonal goat anti-PCSK9, Sigma; monoclonal mouse anti-PCSK9, clone: 2F1, ThermoFisher).

    Techniques: Serum Depletion, Confocal Microscopy

    Overview figure summarizing the content of the manuscript. The figure represents changes in PCSK9, LDLR, ANXA2 and CD36 levels after 20 weeks HFD and in PCSK9 gene knockout mice. The quantity of the colour coded proteins is proportional with the amount found under experimental conditions. Changes in different lipid levels are listed in the tables

    Journal: Nutrition & Metabolism

    Article Title: High fat diet and PCSK9 knockout modulates lipid profile of the liver and changes the expression of lipid homeostasis related genes

    doi: 10.1186/s12986-023-00738-z

    Figure Lengend Snippet: Overview figure summarizing the content of the manuscript. The figure represents changes in PCSK9, LDLR, ANXA2 and CD36 levels after 20 weeks HFD and in PCSK9 gene knockout mice. The quantity of the colour coded proteins is proportional with the amount found under experimental conditions. Changes in different lipid levels are listed in the tables

    Article Snippet: Primary antibodies were incubated overnight at 4 °C in 1:100 dilution (monoclonal mouse anti-CD36, clone: FA6-152, Abcam, UK; monoclonal rabbit anti-LDLR, clone: SJ0197, ThermoFisher; monoclonal rabbit anti-Annexin A2, clone: JA42-30, ThermoFisher; polyclonal goat anti-PCSK9, Sigma; monoclonal mouse anti-PCSK9, clone: 2F1, ThermoFisher).

    Techniques: Gene Knockout

    The primer sequences for qRT-PCR analysis.

    Journal: Foods

    Article Title: The Effects of Anthocyanin-Rich Bilberry Extract on Transintestinal Cholesterol Excretion

    doi: 10.3390/foods10112852

    Figure Lengend Snippet: The primer sequences for qRT-PCR analysis.

    Article Snippet: Goat polyclonal Anti-PCSK9 antibody , Abcam , ab28770.

    Techniques:

    Antibodies used for Western blot.

    Journal: Foods

    Article Title: The Effects of Anthocyanin-Rich Bilberry Extract on Transintestinal Cholesterol Excretion

    doi: 10.3390/foods10112852

    Figure Lengend Snippet: Antibodies used for Western blot.

    Article Snippet: Goat polyclonal Anti-PCSK9 antibody , Abcam , ab28770.

    Techniques: Western Blot